Coding

Part:BBa_K4384004

Designed by: Zike Yao   Group: iGEM22_HK_SSC   (2022-10-09)


5YSS_WT

This cyclase is expected to convert the molecule 5AVA to valerolactam. It is the original cyclase. We have chosen to use this cyclase as it was reported that an ORF encoding CF3HBD from Citrobacter freundii (CF3HBD) has a positive fluorescence signal corresponding to the conversion of 6-ACA to ε-caprolactam, which indicates that it may also bind to 5AVA and convert to valerolactam [1].

References: [1] Yeom, S.-J.; Kim, M.; Kwon, K. K.; Fu, Y.; Rha, E.; Park, S.-H.; Lee, H.; Kim, H.; Lee, D.-H.; Kim, D.-M.; Lee, S.-G. A Synthetic Microbial Biosensor for High-Throughput Screening of Lactam Biocatalysts. Nature Communications 2018, 9 (1). https://doi.org/10.1038/s41467-018-07488-0.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 10
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None